ssr_primer
Primer Details for TRINITY_DN69270_c6_g3_i2 |
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| (A)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAAACGTCGCAAGGTGCTTG | TTTTTAGGATTGGGCCGGGG | Start: 158 |
| Tm | 59.416 | 60.325 | End: 423 |
| Size | 20 | 20 | 266 |
| Primer 2 details |
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| Sequence | GCAAGGTGCTTGTGCTACTT | TTTTTAGGATTGGGCCGGGG | Start: 166 |
| Tm | 59.046 | 60.325 | End: 423 |
| Size | 20 | 20 | 258 |
| Primer 3 details |
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| Sequence | TTAAACGTCGCAAGGTGCTTG | TTTTTAGGATTGGGCCGGGG | Start: 157 |
| Tm | 60.003 | 60.325 | End: 423 |
| Size | 21 | 20 | 267 |
| Primer 4 details |
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| Sequence | CTTTGGACAGAGGGTGATGCT | TTTTTAGGATTGGGCCGGGG | Start: 183 |
| Tm | 59.996 | 60.325 | End: 423 |
| Size | 21 | 20 | 241 |
| Primer 5 details |
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| Sequence | GCGGCGGAAAGTTTTAAACG | TTTTTAGGATTGGGCCGGGG | Start: 144 |
| Tm | 58.953 | 60.325 | End: 423 |
| Size | 20 | 20 | 280 |
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