ssr_primer
Primer Details for TRINITY_DN69276_c3_g1_i2 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAAAGTTGGCTGCTTCCA | TGCTGTGGTGGTTAACTCGG | Start: 330 |
| Tm | 59.814 | 60.25 | End: 501 |
| Size | 20 | 20 | 172 |
| Primer 2 details |
|---|
| Sequence | AGCGCCGTTATGTACAGAGC | TGGAAGCAGCCAACTTTCCT | Start: 148 |
| Tm | 60.528 | 59.814 | End: 349 |
| Size | 20 | 20 | 202 |
| Primer 3 details |
|---|
| Sequence | TGGCTGCTTCCAGAAACTCA | TGCTGTGGTGGTTAACTCGG | Start: 338 |
| Tm | 59.526 | 60.25 | End: 501 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
|---|
| Sequence | CGCCTTCAAACGTGCCTAAA | TGGAAGCAGCCAACTTTCCT | Start: 88 |
| Tm | 59.411 | 59.814 | End: 349 |
| Size | 20 | 20 | 262 |
| Primer 5 details |
|---|
| Sequence | AGGAAAGTTGGCTGCTTCCA | ACTCGGCGTTATGTGTGAGT | Start: 330 |
| Tm | 59.814 | 59.398 | End: 487 |
| Size | 20 | 20 | 158 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAAAGTTGGCTGCTTCCA | TGCTGTGGTGGTTAACTCGG | Start: 330 |
| Tm | 59.814 | 60.25 | End: 501 |
| Size | 20 | 20 | 172 |
| Primer 2 details |
|---|
| Sequence | AGCGCCGTTATGTACAGAGC | TGGAAGCAGCCAACTTTCCT | Start: 148 |
| Tm | 60.528 | 59.814 | End: 349 |
| Size | 20 | 20 | 202 |
| Primer 3 details |
|---|
| Sequence | TGGCTGCTTCCAGAAACTCA | TGCTGTGGTGGTTAACTCGG | Start: 338 |
| Tm | 59.526 | 60.25 | End: 501 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
|---|
| Sequence | CGCCTTCAAACGTGCCTAAA | TGGAAGCAGCCAACTTTCCT | Start: 88 |
| Tm | 59.411 | 59.814 | End: 349 |
| Size | 20 | 20 | 262 |
| Primer 5 details |
|---|
| Sequence | AGGAAAGTTGGCTGCTTCCA | ACTCGGCGTTATGTGTGAGT | Start: 330 |
| Tm | 59.814 | 59.398 | End: 487 |
| Size | 20 | 20 | 158 |
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