ssr_primer
Primer Details for TRINITY_DN69335_c4_g1_i7 |
|---|
| (T)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCACGTCCCTGCTTATGTT | TGAACCCTCTGCAATGAGCC | Start: 2801 |
| Tm | 59.964 | 60.323 | End: 2912 |
| Size | 20 | 20 | 112 |
| Primer 2 details |
|---|
| Sequence | TTCTGTGCCTTGGTTCTCCG | CCTTGGCTGAAAGGTGACGA | Start: 1213 |
| Tm | 60.25 | 60.25 | End: 1424 |
| Size | 20 | 20 | 212 |
| Primer 3 details |
|---|
| Sequence | TGCCTTGGTTCTCCGGAAAT | CCTTGGCTGAAAGGTGACGA | Start: 1218 |
| Tm | 59.596 | 60.25 | End: 1424 |
| Size | 20 | 20 | 207 |
| Primer 4 details |
|---|
| Sequence | CTGCACGTCCCTGCTTATGT | TGAACCCTCTGCAATGAGCC | Start: 2800 |
| Tm | 60.392 | 60.323 | End: 2912 |
| Size | 20 | 20 | 113 |
| Primer 5 details |
|---|
| Sequence | ACCCCTGTCCTTTCCATCCA | CGGAGAACCAAGGCACAGAA | Start: 1132 |
| Tm | 60.478 | 60.25 | End: 1232 |
| Size | 20 | 20 | 101 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCACGTCCCTGCTTATGTT | TGAACCCTCTGCAATGAGCC | Start: 2801 |
| Tm | 59.964 | 60.323 | End: 2912 |
| Size | 20 | 20 | 112 |
| Primer 2 details |
|---|
| Sequence | TTCTGTGCCTTGGTTCTCCG | CCTTGGCTGAAAGGTGACGA | Start: 1213 |
| Tm | 60.25 | 60.25 | End: 1424 |
| Size | 20 | 20 | 212 |
| Primer 3 details |
|---|
| Sequence | TGCCTTGGTTCTCCGGAAAT | CCTTGGCTGAAAGGTGACGA | Start: 1218 |
| Tm | 59.596 | 60.25 | End: 1424 |
| Size | 20 | 20 | 207 |
| Primer 4 details |
|---|
| Sequence | CTGCACGTCCCTGCTTATGT | TGAACCCTCTGCAATGAGCC | Start: 2800 |
| Tm | 60.392 | 60.323 | End: 2912 |
| Size | 20 | 20 | 113 |
| Primer 5 details |
|---|
| Sequence | ACCCCTGTCCTTTCCATCCA | CGGAGAACCAAGGCACAGAA | Start: 1132 |
| Tm | 60.478 | 60.25 | End: 1232 |
| Size | 20 | 20 | 101 |
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