Primer Details for TRINITY_DN69576_c4_g4_i1 |
|---|
| (ATTT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCAAATATTAGAGTCAAATGCA | TCCTCCAAGATGATAACTCGATCG | Start: 35 |
| Tm | 57.228 | 59.784 | End: 134 |
| Size | 24 | 24 | 100 |
| Primer 2 details |
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| Sequence | TGCCAAATATTAGAGTCAAATGCATCA | TCCTCCAAGATGATAACTCGATCG | Start: 35 |
| Tm | 59.932 | 59.784 | End: 134 |
| Size | 27 | 24 | 100 |
| Primer 3 details |
|---|
| Sequence | TGCCAAATATTAGAGTCAAATGCAT | TCCTCCAAGATGATAACTCGATCG | Start: 35 |
| Tm | 57.649 | 59.784 | End: 134 |
| Size | 25 | 24 | 100 |
| Primer 4 details |
|---|
| Sequence | ATGCCAAATATTAGAGTCAAATGCA | TCCTCCAAGATGATAACTCGATCG | Start: 34 |
| Tm | 57.649 | 59.784 | End: 134 |
| Size | 25 | 24 | 101 |
| Primer 5 details |
|---|
| Sequence | TGCCAAATATTAGAGTCAAATGCATC | TCCTCCAAGATGATAACTCGATCG | Start: 35 |
| Tm | 58.572 | 59.784 | End: 134 |
| Size | 26 | 24 | 100 |
(T)26ataaaaaaacccc(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCAAATATTAGAGTCAAATGCA | TCCTCCAAGATGATAACTCGATCG | Start: 35 |
| Tm | 57.228 | 59.784 | End: 134 |
| Size | 24 | 24 | 100 |
| Primer 2 details |
|---|
| Sequence | TGCCAAATATTAGAGTCAAATGCATCA | TCCTCCAAGATGATAACTCGATCG | Start: 35 |
| Tm | 59.932 | 59.784 | End: 134 |
| Size | 27 | 24 | 100 |
| Primer 3 details |
|---|
| Sequence | TGCCAAATATTAGAGTCAAATGCAT | TCCTCCAAGATGATAACTCGATCG | Start: 35 |
| Tm | 57.649 | 59.784 | End: 134 |
| Size | 25 | 24 | 100 |
| Primer 4 details |
|---|
| Sequence | ATGCCAAATATTAGAGTCAAATGCA | TCCTCCAAGATGATAACTCGATCG | Start: 34 |
| Tm | 57.649 | 59.784 | End: 134 |
| Size | 25 | 24 | 101 |
| Primer 5 details |
|---|
| Sequence | TGCCAAATATTAGAGTCAAATGCATC | TCCTCCAAGATGATAACTCGATCG | Start: 35 |
| Tm | 58.572 | 59.784 | End: 134 |
| Size | 26 | 24 | 100 |
