ssr_primer
Primer Details for TRINITY_DN69656_c4_g2_i3 |
|---|
| (T)10a(T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACGGAAAAAGGCCCCTAGG | TGTAAGCAAGCAGTGACGGT | Start: 313 |
| Tm | 59.961 | 59.894 | End: 457 |
| Size | 20 | 20 | 145 |
| Primer 2 details |
|---|
| Sequence | TGAAGGGGCTCCTCTTACGA | ATCGAGTGCTGCCTTGGTAC | Start: 33 |
| Tm | 59.959 | 60.108 | End: 292 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | TGAAGGGGCTCCTCTTACGA | CACCAACAATCGAGTGCTGC | Start: 33 |
| Tm | 59.959 | 60.11 | End: 300 |
| Size | 20 | 20 | 268 |
| Primer 4 details |
|---|
| Sequence | GTACCAAGGCAGCACTCGAT | TGTAAGCAAGCAGTGACGGT | Start: 273 |
| Tm | 60.108 | 59.894 | End: 457 |
| Size | 20 | 20 | 185 |
| Primer 5 details |
|---|
| Sequence | GCAGCACTCGATTGTTGGTG | TGTAAGCAAGCAGTGACGGT | Start: 281 |
| Tm | 60.11 | 59.894 | End: 457 |
| Size | 20 | 20 | 177 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACGGAAAAAGGCCCCTAGG | TGTAAGCAAGCAGTGACGGT | Start: 313 |
| Tm | 59.961 | 59.894 | End: 457 |
| Size | 20 | 20 | 145 |
| Primer 2 details |
|---|
| Sequence | TGAAGGGGCTCCTCTTACGA | ATCGAGTGCTGCCTTGGTAC | Start: 33 |
| Tm | 59.959 | 60.108 | End: 292 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | TGAAGGGGCTCCTCTTACGA | CACCAACAATCGAGTGCTGC | Start: 33 |
| Tm | 59.959 | 60.11 | End: 300 |
| Size | 20 | 20 | 268 |
| Primer 4 details |
|---|
| Sequence | GTACCAAGGCAGCACTCGAT | TGTAAGCAAGCAGTGACGGT | Start: 273 |
| Tm | 60.108 | 59.894 | End: 457 |
| Size | 20 | 20 | 185 |
| Primer 5 details |
|---|
| Sequence | GCAGCACTCGATTGTTGGTG | TGTAAGCAAGCAGTGACGGT | Start: 281 |
| Tm | 60.11 | 59.894 | End: 457 |
| Size | 20 | 20 | 177 |
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