ssr_primer
Primer Details for TRINITY_DN69899_c4_g2_i2 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTCGGTGCTTCCTTGTTT | TAAACCCGTGGGAAGATGCC | Start: 751 |
| Tm | 60.107 | 60.035 | End: 961 |
| Size | 20 | 20 | 211 |
| Primer 2 details |
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| Sequence | ATGGTCGGTGCTTCCTTGTT | TAAACCCGTGGGAAGATGCC | Start: 750 |
| Tm | 59.89 | 60.035 | End: 961 |
| Size | 20 | 20 | 212 |
| Primer 3 details |
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| Sequence | AATGGTCGGTGCTTCCTTGT | TAAACCCGTGGGAAGATGCC | Start: 749 |
| Tm | 59.89 | 60.035 | End: 961 |
| Size | 20 | 20 | 213 |
| Primer 4 details |
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| Sequence | CACCAGCAAACGACAGCAAA | AAACAAGGAAGCACCGACCA | Start: 657 |
| Tm | 59.9 | 60.107 | End: 770 |
| Size | 20 | 20 | 114 |
| Primer 5 details |
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| Sequence | CACCAGCAAACGACAGCAAA | ACAAGGAAGCACCGACCATT | Start: 657 |
| Tm | 59.9 | 59.89 | End: 768 |
| Size | 20 | 20 | 112 |
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