ssr_primer
Primer Details for TRINITY_DN70009_c6_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGAATGGGTGGGATGAGC | TGCAAGAGAGACCACGGAAC | Start: 461 |
| Tm | 60.033 | 59.966 | End: 588 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
|---|
| Sequence | TGAAAAGGAGCGCAAACTGC | AAGCGGCAATCTTCGGTACA | Start: 1530 |
| Tm | 59.969 | 60.037 | End: 1693 |
| Size | 20 | 20 | 164 |
| Primer 3 details |
|---|
| Sequence | ACTGCTCAAAGACGCCGTAA | AAGCGGCAATCTTCGGTACA | Start: 1545 |
| Tm | 59.967 | 60.037 | End: 1693 |
| Size | 20 | 20 | 149 |
| Primer 4 details |
|---|
| Sequence | AACTGCTCAAAGACGCCGTA | AAGCGGCAATCTTCGGTACA | Start: 1544 |
| Tm | 59.967 | 60.037 | End: 1693 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
|---|
| Sequence | TTGGAATGGGTGGGATGAGC | ACCATGCAAGAGAGACCACG | Start: 461 |
| Tm | 60.033 | 60.037 | End: 592 |
| Size | 20 | 20 | 132 |
(TAA)7ttgattttcttcattttatttttaattaaattttaa(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGAATGGGTGGGATGAGC | TGCAAGAGAGACCACGGAAC | Start: 461 |
| Tm | 60.033 | 59.966 | End: 588 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
|---|
| Sequence | TGAAAAGGAGCGCAAACTGC | AAGCGGCAATCTTCGGTACA | Start: 1530 |
| Tm | 59.969 | 60.037 | End: 1693 |
| Size | 20 | 20 | 164 |
| Primer 3 details |
|---|
| Sequence | ACTGCTCAAAGACGCCGTAA | AAGCGGCAATCTTCGGTACA | Start: 1545 |
| Tm | 59.967 | 60.037 | End: 1693 |
| Size | 20 | 20 | 149 |
| Primer 4 details |
|---|
| Sequence | AACTGCTCAAAGACGCCGTA | AAGCGGCAATCTTCGGTACA | Start: 1544 |
| Tm | 59.967 | 60.037 | End: 1693 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
|---|
| Sequence | TTGGAATGGGTGGGATGAGC | ACCATGCAAGAGAGACCACG | Start: 461 |
| Tm | 60.033 | 60.037 | End: 592 |
| Size | 20 | 20 | 132 |
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