ssr_primer
Primer Details for TRINITY_DN70022_c3_g1_i12 |
|---|
| (A)28: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTGGCGGGAAAGTGGATTA | GGGGCGCCAAGGTAAACTAA | Start: 734 |
| Tm | 60.035 | 60.323 | End: 980 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
|---|
| Sequence | TTAGTTTACCTTGGCGCCCC | GCCATAACACACCCTCCTCC | Start: 961 |
| Tm | 60.323 | 60.107 | End: 1185 |
| Size | 20 | 20 | 225 |
| Primer 3 details |
|---|
| Sequence | AGAGAAAGTTGCCAACGCCT | GGCTCTCTACCTGCAAAGCT | Start: 1513 |
| Tm | 60.179 | 59.748 | End: 1667 |
| Size | 20 | 20 | 155 |
| Primer 4 details |
|---|
| Sequence | AGAGAAAGTTGCCAACGCCT | AGGCTCTCTACCTGCAAAGC | Start: 1513 |
| Tm | 60.179 | 59.748 | End: 1668 |
| Size | 20 | 20 | 156 |
| Primer 5 details |
|---|
| Sequence | AAGTTGCCAACGCCTAGTGT | GGCTCTCTACCTGCAAAGCT | Start: 1518 |
| Tm | 60.179 | 59.748 | End: 1667 |
| Size | 20 | 20 | 150 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTGGCGGGAAAGTGGATTA | GGGGCGCCAAGGTAAACTAA | Start: 734 |
| Tm | 60.035 | 60.323 | End: 980 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
|---|
| Sequence | TTAGTTTACCTTGGCGCCCC | GCCATAACACACCCTCCTCC | Start: 961 |
| Tm | 60.323 | 60.107 | End: 1185 |
| Size | 20 | 20 | 225 |
| Primer 3 details |
|---|
| Sequence | AGAGAAAGTTGCCAACGCCT | GGCTCTCTACCTGCAAAGCT | Start: 1513 |
| Tm | 60.179 | 59.748 | End: 1667 |
| Size | 20 | 20 | 155 |
| Primer 4 details |
|---|
| Sequence | AGAGAAAGTTGCCAACGCCT | AGGCTCTCTACCTGCAAAGC | Start: 1513 |
| Tm | 60.179 | 59.748 | End: 1668 |
| Size | 20 | 20 | 156 |
| Primer 5 details |
|---|
| Sequence | AAGTTGCCAACGCCTAGTGT | GGCTCTCTACCTGCAAAGCT | Start: 1518 |
| Tm | 60.179 | 59.748 | End: 1667 |
| Size | 20 | 20 | 150 |
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