ssr_primer
Primer Details for TRINITY_DN70040_c8_g1_i1 |
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| (T)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACCGGGCTCTCTTCAATGC | CGGCAATTCTTTTTGGTTTTGGT | Start: 12 |
| Tm | 59.822 | 59.375 | End: 187 |
| Size | 20 | 23 | 176 |
| Primer 2 details |
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| Sequence | TACCGGGCTCTCTTCAATGC | ACGGCAATTCTTTTTGGTTTTGG | Start: 12 |
| Tm | 59.822 | 59.375 | End: 188 |
| Size | 20 | 23 | 177 |
| Primer 3 details |
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| Sequence | GATCGTCCCTACAAGCGCTT | CGGCAATTCTTTTTGGTTTTGGT | Start: 53 |
| Tm | 60.179 | 59.375 | End: 187 |
| Size | 20 | 23 | 135 |
| Primer 4 details |
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| Sequence | GATCGTCCCTACAAGCGCTT | ACGGCAATTCTTTTTGGTTTTGG | Start: 53 |
| Tm | 60.179 | 59.375 | End: 188 |
| Size | 20 | 23 | 136 |
| Primer 5 details |
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| Sequence | TTTTTGTCGTTTTCCCCGCG | CGGCAATTCTTTTTGGTTTTGGT | Start: 32 |
| Tm | 60.249 | 59.375 | End: 187 |
| Size | 20 | 23 | 156 |
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