ssr_primer
Primer Details for TRINITY_DN70352_c3_g4_i3 |
|---|
| (A)19: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCTTCCTGCTAAACGCGGT | CCCCGTGATCCCATGTTGAA | Start: 202 |
| Tm | 59.966 | 60.035 | End: 428 |
| Size | 20 | 20 | 227 |
| Primer 2 details |
|---|
| Sequence | TTCTTCCTGCTAAACGCGGT | TTCCCCGTGATCCCATGTTG | Start: 202 |
| Tm | 59.966 | 60.035 | End: 430 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | CAACATGGGATCACGGGGAA | GTCATTGGCAGAGGTCGGAA | Start: 411 |
| Tm | 60.035 | 60.036 | End: 642 |
| Size | 20 | 20 | 232 |
| Primer 4 details |
|---|
| Sequence | TTCAACATGGGATCACGGGG | GTCATTGGCAGAGGTCGGAA | Start: 409 |
| Tm | 60.035 | 60.036 | End: 642 |
| Size | 20 | 20 | 234 |
| Primer 5 details |
|---|
| Sequence | TTCCGACCTCTGCCAATGAC | TAGGTACCAACGCACGACAC | Start: 623 |
| Tm | 60.036 | 60.039 | End: 877 |
| Size | 20 | 20 | 255 |
(TTTTC)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCTTCCTGCTAAACGCGGT | CCCCGTGATCCCATGTTGAA | Start: 202 |
| Tm | 59.966 | 60.035 | End: 428 |
| Size | 20 | 20 | 227 |
| Primer 2 details |
|---|
| Sequence | TTCTTCCTGCTAAACGCGGT | TTCCCCGTGATCCCATGTTG | Start: 202 |
| Tm | 59.966 | 60.035 | End: 430 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | CAACATGGGATCACGGGGAA | GTCATTGGCAGAGGTCGGAA | Start: 411 |
| Tm | 60.035 | 60.036 | End: 642 |
| Size | 20 | 20 | 232 |
| Primer 4 details |
|---|
| Sequence | TTCAACATGGGATCACGGGG | GTCATTGGCAGAGGTCGGAA | Start: 409 |
| Tm | 60.035 | 60.036 | End: 642 |
| Size | 20 | 20 | 234 |
| Primer 5 details |
|---|
| Sequence | TTCCGACCTCTGCCAATGAC | TAGGTACCAACGCACGACAC | Start: 623 |
| Tm | 60.036 | 60.039 | End: 877 |
| Size | 20 | 20 | 255 |
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