Primer Details for TRINITY_DN70544_c9_g2_i2 | |||
|---|---|---|---|
| (A)20: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAAACAGGTGAGATTGTGTCG | TTGATCACAAATGAACTGTTACCCT | Start: 136 |
| Tm | 58.869 | 58.932 | End: 235 |
| Size | 22 | 25 | 100 |
| Primer 2 details | |||
| Sequence | TCAAACAGGTGAGATTGTGTCGA | TTGATCACAAATGAACTGTTACCCT | Start: 136 |
| Tm | 60.181 | 58.932 | End: 235 |
| Size | 23 | 25 | 100 |
| Primer 3 details | |||
| Sequence | ATCAAACAGGTGAGATTGTGTCG | TGATCACAAATGAACTGTTACCCT | Start: 135 |
| Tm | 59.252 | 58.37 | End: 234 |
| Size | 23 | 24 | 100 |
| Primer 4 details | |||
| Sequence | TCAAACAGGTGAGATTGTGTCG | TTGATCACAAATGAACTGTTACCC | Start: 136 |
| Tm | 58.869 | 57.674 | End: 235 |
| Size | 22 | 24 | 100 |
| Primer 5 details | |||
| Sequence | TCAAACAGGTGAGATTGTGTCGA | TTGATCACAAATGAACTGTTACCC | Start: 136 |
| Tm | 60.181 | 57.674 | End: 235 |
| Size | 23 | 24 | 100 |
