ssr_primer
Primer Details for TRINITY_DN70879_c4_g2_i1 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTTACGCTGGTTCGTACGG | CTCGGACGGGGATGGAAAAA | Start: 670 |
| Tm | 60.11 | 60.036 | End: 806 |
| Size | 20 | 20 | 137 |
| Primer 2 details |
|---|
| Sequence | TGATGTTGGAACGGTGCTCA | TTCACGAGGGAAACGACGAG | Start: 354 |
| Tm | 59.892 | 60.04 | End: 465 |
| Size | 20 | 20 | 112 |
| Primer 3 details |
|---|
| Sequence | CTCGTCGTTTCCCTCGTGAA | CCGTACGAACCAGCGTAACT | Start: 446 |
| Tm | 60.04 | 60.11 | End: 689 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | AGTTACGCTGGTTCGTACGG | GTTATTGCCGAGAGCCCCTT | Start: 670 |
| Tm | 60.11 | 60.107 | End: 839 |
| Size | 20 | 20 | 170 |
| Primer 5 details |
|---|
| Sequence | TGTGCAACCTTGGGATGGAG | CTCGGACGGGGATGGAAAAA | Start: 652 |
| Tm | 60.251 | 60.036 | End: 806 |
| Size | 20 | 20 | 155 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTTACGCTGGTTCGTACGG | CTCGGACGGGGATGGAAAAA | Start: 670 |
| Tm | 60.11 | 60.036 | End: 806 |
| Size | 20 | 20 | 137 |
| Primer 2 details |
|---|
| Sequence | TGATGTTGGAACGGTGCTCA | TTCACGAGGGAAACGACGAG | Start: 354 |
| Tm | 59.892 | 60.04 | End: 465 |
| Size | 20 | 20 | 112 |
| Primer 3 details |
|---|
| Sequence | CTCGTCGTTTCCCTCGTGAA | CCGTACGAACCAGCGTAACT | Start: 446 |
| Tm | 60.04 | 60.11 | End: 689 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | AGTTACGCTGGTTCGTACGG | GTTATTGCCGAGAGCCCCTT | Start: 670 |
| Tm | 60.11 | 60.107 | End: 839 |
| Size | 20 | 20 | 170 |
| Primer 5 details |
|---|
| Sequence | TGTGCAACCTTGGGATGGAG | CTCGGACGGGGATGGAAAAA | Start: 652 |
| Tm | 60.251 | 60.036 | End: 806 |
| Size | 20 | 20 | 155 |
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