ssr_primer
Primer Details for TRINITY_DN71033_c0_g1_i2 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATTTGCAAGGTCGCCCAAC | GTACGCACGAAGGACTTGGA | Start: 131 |
| Tm | 59.967 | 60.039 | End: 261 |
| Size | 20 | 20 | 131 |
| Primer 2 details |
|---|
| Sequence | AATTTGCAAGGTCGCCCAAC | AGGACTTGGAGTAGACCGCT | Start: 131 |
| Tm | 59.967 | 59.96 | End: 251 |
| Size | 20 | 20 | 121 |
| Primer 3 details |
|---|
| Sequence | CTGTCATTGACCGGGCTCTT | GTACGCACGAAGGACTTGGA | Start: 158 |
| Tm | 60.036 | 60.039 | End: 261 |
| Size | 20 | 20 | 104 |
| Primer 4 details |
|---|
| Sequence | TGCCCCCACTGAGATCCTTA | AAGAGCCCGGTCAATGACAG | Start: 36 |
| Tm | 59.956 | 60.036 | End: 177 |
| Size | 20 | 20 | 142 |
| Primer 5 details |
|---|
| Sequence | CCGAGTTTTGTTGCCAGAGC | GACTCAGCGCCCAAAAGTTG | Start: 328 |
| Tm | 60.04 | 60.04 | End: 594 |
| Size | 20 | 20 | 267 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATTTGCAAGGTCGCCCAAC | GTACGCACGAAGGACTTGGA | Start: 131 |
| Tm | 59.967 | 60.039 | End: 261 |
| Size | 20 | 20 | 131 |
| Primer 2 details |
|---|
| Sequence | AATTTGCAAGGTCGCCCAAC | AGGACTTGGAGTAGACCGCT | Start: 131 |
| Tm | 59.967 | 59.96 | End: 251 |
| Size | 20 | 20 | 121 |
| Primer 3 details |
|---|
| Sequence | CTGTCATTGACCGGGCTCTT | GTACGCACGAAGGACTTGGA | Start: 158 |
| Tm | 60.036 | 60.039 | End: 261 |
| Size | 20 | 20 | 104 |
| Primer 4 details |
|---|
| Sequence | TGCCCCCACTGAGATCCTTA | AAGAGCCCGGTCAATGACAG | Start: 36 |
| Tm | 59.956 | 60.036 | End: 177 |
| Size | 20 | 20 | 142 |
| Primer 5 details |
|---|
| Sequence | CCGAGTTTTGTTGCCAGAGC | GACTCAGCGCCCAAAAGTTG | Start: 328 |
| Tm | 60.04 | 60.04 | End: 594 |
| Size | 20 | 20 | 267 |
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