ssr_primer
Primer Details for TRINITY_DN71115_c5_g2_i1 |
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| (A)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATCAAAGGACGGGCACGAA | AGCATTGAGAAGCGGGCTAG | Start: 73 |
| Tm | 60.251 | 60.179 | End: 176 |
| Size | 20 | 20 | 104 |
| Primer 2 details |
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| Sequence | AAATCAAAGGACGGGCACGA | AGCATTGAGAAGCGGGCTAG | Start: 72 |
| Tm | 60.251 | 60.179 | End: 176 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
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| Sequence | CAAAGGACGGGCACGAAGTA | AGCATTGAGAAGCGGGCTAG | Start: 76 |
| Tm | 60.32 | 60.179 | End: 176 |
| Size | 20 | 20 | 101 |
| Primer 4 details |
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| Sequence | AATCAAAGGACGGGCACGAA | AAGCATTGAGAAGCGGGCTA | Start: 73 |
| Tm | 60.251 | 59.747 | End: 177 |
| Size | 20 | 20 | 105 |
| Primer 5 details |
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| Sequence | AAATCAAAGGACGGGCACGA | AAGCATTGAGAAGCGGGCTA | Start: 72 |
| Tm | 60.251 | 59.747 | End: 177 |
| Size | 20 | 20 | 106 |
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