Primer Details for TRINITY_DN71257_c4_g1_i1 | |||
|---|---|---|---|
| (A)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGCATTTGTTACGCCAAA | TGCGATTCATTTTGAATGGAAGT | Start: 9 |
| Tm | 59.331 | 57.61 | End: 137 |
| Size | 20 | 23 | 129 |
| Primer 2 details | |||
| Sequence | ACGGCATTTGTTACGCCAAA | TGCGATTCATTTTGAATGGAAGTT | Start: 9 |
| Tm | 59.331 | 58.218 | End: 137 |
| Size | 20 | 24 | 129 |
| Primer 3 details | |||
| Sequence | ACGGCATTTGTTACGCCAAA | TTGCGATTCATTTTGAATGGAAGT | Start: 9 |
| Tm | 59.331 | 58.218 | End: 138 |
| Size | 20 | 24 | 130 |
| Primer 4 details | |||
| Sequence | ACGGCATTTGTTACGCCAAAT | TGCGATTCATTTTGAATGGAAGT | Start: 9 |
| Tm | 59.729 | 57.61 | End: 137 |
| Size | 21 | 23 | 129 |
| Primer 5 details | |||
| Sequence | ACGGCATTTGTTACGCCAAA | TGCGATTCATTTTGAATGGAAGTTT | Start: 9 |
| Tm | 59.331 | 58.778 | End: 137 |
| Size | 20 | 25 | 129 |
