ssr_primer
Primer Details for TRINITY_DN71276_c2_g3_i6 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTCTCGCCCTTGGTAAGT | TTGGCGAAACCGTGATCGTA | Start: 1741 |
| Tm | 60.036 | 60.038 | End: 1969 |
| Size | 20 | 20 | 229 |
| Primer 2 details |
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| Sequence | GCTTCTCGCCCTTGGTAAGT | ACAAAGTTGGCGAAACCGTG | Start: 1741 |
| Tm | 60.036 | 59.9 | End: 1975 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
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| Sequence | AACCCTGTCTTCCTCCCTGT | ATTGGCTCCGGGCTGTTAAA | Start: 1295 |
| Tm | 60.104 | 59.961 | End: 1451 |
| Size | 20 | 20 | 157 |
| Primer 4 details |
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| Sequence | TCCAGGTGTCGTGGAAAGTG | ATTGGCTCCGGGCTGTTAAA | Start: 1194 |
| Tm | 59.894 | 59.961 | End: 1451 |
| Size | 20 | 20 | 258 |
| Primer 5 details |
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| Sequence | TACGATCACGGTTTCGCCAA | GTCGTGCTTTCTCCCGATCA | Start: 1950 |
| Tm | 60.038 | 60.109 | End: 2130 |
| Size | 20 | 20 | 181 |
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