ssr_primer
Primer Details for TRINITY_DN71358_c12_g1_i1 |
|---|
| (A)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGACTATTGTCCGACCTCCG | TCTGCACGAGGGCACTTTAG | Start: 402 |
| Tm | 59.971 | 60.037 | End: 584 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
|---|
| Sequence | CTAAAGTGCCCTCGTGCAGA | TCTCGAAAAACTGCGTCCGA | Start: 565 |
| Tm | 60.037 | 59.969 | End: 783 |
| Size | 20 | 20 | 219 |
| Primer 3 details |
|---|
| Sequence | ACCTCCCTCGTATGGAACCA | TCTGCACGAGGGCACTTTAG | Start: 369 |
| Tm | 59.958 | 60.037 | End: 584 |
| Size | 20 | 20 | 216 |
| Primer 4 details |
|---|
| Sequence | GGTCGCGTTAATTTTCCGGG | TCTGCACGAGGGCACTTTAG | Start: 468 |
| Tm | 59.903 | 60.037 | End: 584 |
| Size | 20 | 20 | 117 |
| Primer 5 details |
|---|
| Sequence | GACCTCCGATGACCACGTAC | TCTGCACGAGGGCACTTTAG | Start: 414 |
| Tm | 59.9 | 60.037 | End: 584 |
| Size | 20 | 20 | 171 |
(A)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGACTATTGTCCGACCTCCG | TCTGCACGAGGGCACTTTAG | Start: 402 |
| Tm | 59.971 | 60.037 | End: 584 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
|---|
| Sequence | CTAAAGTGCCCTCGTGCAGA | TCTCGAAAAACTGCGTCCGA | Start: 565 |
| Tm | 60.037 | 59.969 | End: 783 |
| Size | 20 | 20 | 219 |
| Primer 3 details |
|---|
| Sequence | ACCTCCCTCGTATGGAACCA | TCTGCACGAGGGCACTTTAG | Start: 369 |
| Tm | 59.958 | 60.037 | End: 584 |
| Size | 20 | 20 | 216 |
| Primer 4 details |
|---|
| Sequence | GGTCGCGTTAATTTTCCGGG | TCTGCACGAGGGCACTTTAG | Start: 468 |
| Tm | 59.903 | 60.037 | End: 584 |
| Size | 20 | 20 | 117 |
| Primer 5 details |
|---|
| Sequence | GACCTCCGATGACCACGTAC | TCTGCACGAGGGCACTTTAG | Start: 414 |
| Tm | 59.9 | 60.037 | End: 584 |
| Size | 20 | 20 | 171 |
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