Primer Details for TRINITY_DN71496_c1_g3_i1 |
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| (G)23tgagactgctgcgctgaagaaaaacgccctatgatcattcgaatgttgccagattcccactcaggaa(T)11caagaacgttatgaatgtttttccttgaactttgtagttttgatcattgtatagg(T)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGACTGCTGCGCTGAAGAAA | AGTTCAAGGAAAAACATTCATAACGT | Start: 67 |
| Tm | 60.25 | 58.287 | End: 173 |
| Size | 20 | 26 | 107 |
| Primer 2 details |
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| Sequence | TGCTGCGCTGAAGAAAAACG | AGTTCAAGGAAAAACATTCATAACGT | Start: 71 |
| Tm | 60.317 | 58.287 | End: 173 |
| Size | 20 | 26 | 103 |
| Primer 3 details |
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| Sequence | AGACTGCTGCGCTGAAGAAA | TGATCAAAACTACAAAGTTCAAGGA | Start: 67 |
| Tm | 60.25 | 57.126 | End: 188 |
| Size | 20 | 25 | 122 |
| Primer 4 details |
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| Sequence | AGACTGCTGCGCTGAAGAAA | GTTCAAGGAAAAACATTCATAACGT | Start: 67 |
| Tm | 60.25 | 57.076 | End: 172 |
| Size | 20 | 25 | 106 |
| Primer 5 details |
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| Sequence | AGACTGCTGCGCTGAAGAAA | AGGAAAAACATTCATAACGTTCTTGA | Start: 67 |
| Tm | 60.25 | 58.063 | End: 167 |
| Size | 20 | 26 | 101 |
