Primer Details for TRINITY_DN71783_c4_g5_i1 | |||
|---|---|---|---|
| (A)19tagttt(A)10caaaaaaa(T)26: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGAAAAACACCGTTAAACAGAA | TTTTTGGGAGCAAATGGGGC | Start: 123 |
| Tm | 57.253 | 59.6 | End: 240 |
| Size | 24 | 20 | 118 |
| Primer 2 details | |||
| Sequence | AGAGAAAAACACCGTTAAACAGAA | TTTTGGGAGCAAATGGGGCT | Start: 123 |
| Tm | 57.253 | 60.474 | End: 239 |
| Size | 24 | 20 | 117 |
| Primer 3 details | |||
| Sequence | AGAAAAACACCGTTAAACAGAAAGT | TTTTTGGGAGCAAATGGGGC | Start: 125 |
| Tm | 58.084 | 59.6 | End: 240 |
| Size | 25 | 20 | 116 |
| Primer 4 details | |||
| Sequence | AGAGAAAAACACCGTTAAACAGAA | GGCTCCGGAGTCAAACTGTA | Start: 123 |
| Tm | 57.253 | 59.393 | End: 223 |
| Size | 24 | 20 | 101 |
| Primer 5 details | |||
| Sequence | AGAAAAACACCGTTAAACAGAAAGT | TTTTGGGAGCAAATGGGGCT | Start: 125 |
| Tm | 58.084 | 60.474 | End: 239 |
| Size | 25 | 20 | 115 |
