ssr_primer
Primer Details for TRINITY_DN71876_c1_g2_i2 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTTCAAAGACGGTGGAGCT | CGCCATGGGACCTGTTACTT | Start: 891 |
| Tm | 59.965 | 60.035 | End: 1097 |
| Size | 20 | 20 | 207 |
| Primer 2 details |
|---|
| Sequence | AAGTAACAGGTCCCATGGCG | TGCAGCCGCATTCTCTGTTA | Start: 1078 |
| Tm | 60.035 | 60.036 | End: 1323 |
| Size | 20 | 20 | 246 |
| Primer 3 details |
|---|
| Sequence | CCTTCAAAGACGGTGGAGCT | AAATCGCCATGGGACCTGTT | Start: 891 |
| Tm | 59.965 | 59.96 | End: 1101 |
| Size | 20 | 20 | 211 |
| Primer 4 details |
|---|
| Sequence | CCTTCAAAGACGGTGGAGCT | AAAATCGCCATGGGACCTGT | Start: 891 |
| Tm | 59.965 | 59.96 | End: 1102 |
| Size | 20 | 20 | 212 |
| Primer 5 details |
|---|
| Sequence | ACAGGTCCCATGGCGATTTT | TGCAGCCGCATTCTCTGTTA | Start: 1083 |
| Tm | 59.96 | 60.036 | End: 1323 |
| Size | 20 | 20 | 241 |
(A)20(GAA)(AGAA)4: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTTCAAAGACGGTGGAGCT | CGCCATGGGACCTGTTACTT | Start: 891 |
| Tm | 59.965 | 60.035 | End: 1097 |
| Size | 20 | 20 | 207 |
| Primer 2 details |
|---|
| Sequence | AAGTAACAGGTCCCATGGCG | TGCAGCCGCATTCTCTGTTA | Start: 1078 |
| Tm | 60.035 | 60.036 | End: 1323 |
| Size | 20 | 20 | 246 |
| Primer 3 details |
|---|
| Sequence | CCTTCAAAGACGGTGGAGCT | AAATCGCCATGGGACCTGTT | Start: 891 |
| Tm | 59.965 | 59.96 | End: 1101 |
| Size | 20 | 20 | 211 |
| Primer 4 details |
|---|
| Sequence | CCTTCAAAGACGGTGGAGCT | AAAATCGCCATGGGACCTGT | Start: 891 |
| Tm | 59.965 | 59.96 | End: 1102 |
| Size | 20 | 20 | 212 |
| Primer 5 details |
|---|
| Sequence | ACAGGTCCCATGGCGATTTT | TGCAGCCGCATTCTCTGTTA | Start: 1083 |
| Tm | 59.96 | 60.036 | End: 1323 |
| Size | 20 | 20 | 241 |
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