ssr_primer
Primer Details for TRINITY_DN71925_c4_g2_i1 |
|---|
| (G)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAGACCCCTCTTGTCGACC | ATCGGCCGCCATTTTGAAAC | Start: 295 |
| Tm | 59.753 | 60.109 | End: 415 |
| Size | 20 | 20 | 121 |
| Primer 2 details |
|---|
| Sequence | GGAAGACCCCTCTTGTCGAC | ATCGGCCGCCATTTTGAAAC | Start: 294 |
| Tm | 59.753 | 60.109 | End: 415 |
| Size | 20 | 20 | 122 |
| Primer 3 details |
|---|
| Sequence | GTTTCAAAATGGCGGCCGAT | TGATCTTCAATCAGCCGCCA | Start: 396 |
| Tm | 60.109 | 59.747 | End: 571 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
|---|
| Sequence | GAAGACCCCTCTTGTCGACC | ACTCTAATCGGCCGCCATTT | Start: 295 |
| Tm | 59.753 | 59.82 | End: 421 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | GAAGACCCCTCTTGTCGACC | AACTCTAATCGGCCGCCATT | Start: 295 |
| Tm | 59.753 | 59.82 | End: 422 |
| Size | 20 | 20 | 128 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAGACCCCTCTTGTCGACC | ATCGGCCGCCATTTTGAAAC | Start: 295 |
| Tm | 59.753 | 60.109 | End: 415 |
| Size | 20 | 20 | 121 |
| Primer 2 details |
|---|
| Sequence | GGAAGACCCCTCTTGTCGAC | ATCGGCCGCCATTTTGAAAC | Start: 294 |
| Tm | 59.753 | 60.109 | End: 415 |
| Size | 20 | 20 | 122 |
| Primer 3 details |
|---|
| Sequence | GTTTCAAAATGGCGGCCGAT | TGATCTTCAATCAGCCGCCA | Start: 396 |
| Tm | 60.109 | 59.747 | End: 571 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
|---|
| Sequence | GAAGACCCCTCTTGTCGACC | ACTCTAATCGGCCGCCATTT | Start: 295 |
| Tm | 59.753 | 59.82 | End: 421 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | GAAGACCCCTCTTGTCGACC | AACTCTAATCGGCCGCCATT | Start: 295 |
| Tm | 59.753 | 59.82 | End: 422 |
| Size | 20 | 20 | 128 |
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