ssr_primer
Primer Details for TRINITY_DN71981_c3_g1_i2 |
|---|
| (T)24: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTACTCCGCGACGTGTTTCA | TTGCGACACGTTGACACAAC | Start: 1020 |
| Tm | 59.969 | 59.904 | End: 1160 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
|---|
| Sequence | TTACTCCGCGACGTGTTTCA | AGGGCTAGCTGCATTTCCAG | Start: 1020 |
| Tm | 59.969 | 60.107 | End: 1197 |
| Size | 20 | 20 | 178 |
| Primer 3 details |
|---|
| Sequence | TTACTCCGCGACGTGTTTCA | GGGCTAGCTGCATTTCCAGA | Start: 1020 |
| Tm | 59.969 | 60.107 | End: 1196 |
| Size | 20 | 20 | 177 |
| Primer 4 details |
|---|
| Sequence | TTACTCCGCGACGTGTTTCA | TCCAGGGCTAGCTGCATTTC | Start: 1020 |
| Tm | 59.969 | 60.107 | End: 1200 |
| Size | 20 | 20 | 181 |
| Primer 5 details |
|---|
| Sequence | AGCGCATTAACCGTTACCCA | GTGTCTTAAACGCCTTCGCC | Start: 574 |
| Tm | 60.036 | 59.835 | End: 764 |
| Size | 20 | 20 | 191 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTACTCCGCGACGTGTTTCA | TTGCGACACGTTGACACAAC | Start: 1020 |
| Tm | 59.969 | 59.904 | End: 1160 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
|---|
| Sequence | TTACTCCGCGACGTGTTTCA | AGGGCTAGCTGCATTTCCAG | Start: 1020 |
| Tm | 59.969 | 60.107 | End: 1197 |
| Size | 20 | 20 | 178 |
| Primer 3 details |
|---|
| Sequence | TTACTCCGCGACGTGTTTCA | GGGCTAGCTGCATTTCCAGA | Start: 1020 |
| Tm | 59.969 | 60.107 | End: 1196 |
| Size | 20 | 20 | 177 |
| Primer 4 details |
|---|
| Sequence | TTACTCCGCGACGTGTTTCA | TCCAGGGCTAGCTGCATTTC | Start: 1020 |
| Tm | 59.969 | 60.107 | End: 1200 |
| Size | 20 | 20 | 181 |
| Primer 5 details |
|---|
| Sequence | AGCGCATTAACCGTTACCCA | GTGTCTTAAACGCCTTCGCC | Start: 574 |
| Tm | 60.036 | 59.835 | End: 764 |
| Size | 20 | 20 | 191 |
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