ssr_primer
Primer Details for TRINITY_DN72004_c1_g1_i9 |
|---|
| (T)11(G)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTTGTCCGTGAGACCGAG | CGTAGGTCTCCTTCCCTCGA | Start: 256 |
| Tm | 59.968 | 60.107 | End: 453 |
| Size | 20 | 20 | 198 |
| Primer 2 details |
|---|
| Sequence | TTGTTGTCCGTGAGACCGAG | TCGTAGGTCTCCTTCCCTCG | Start: 256 |
| Tm | 59.968 | 60.107 | End: 454 |
| Size | 20 | 20 | 199 |
| Primer 3 details |
|---|
| Sequence | GTAAGGGCTTCGGCAGATGT | CTCGGTCTCACGGACAACAA | Start: 37 |
| Tm | 60.108 | 59.968 | End: 275 |
| Size | 20 | 20 | 239 |
| Primer 4 details |
|---|
| Sequence | TGTAAGGGCTTCGGCAGATG | CTCGGTCTCACGGACAACAA | Start: 36 |
| Tm | 60.108 | 59.968 | End: 275 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
|---|
| Sequence | TTGTTGTCCGTGAGACCGAG | GCTGAATGTACCACGCCTCT | Start: 256 |
| Tm | 59.968 | 60.108 | End: 393 |
| Size | 20 | 20 | 138 |
(T)26gtttgt(G)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTTGTCCGTGAGACCGAG | CGTAGGTCTCCTTCCCTCGA | Start: 256 |
| Tm | 59.968 | 60.107 | End: 453 |
| Size | 20 | 20 | 198 |
| Primer 2 details |
|---|
| Sequence | TTGTTGTCCGTGAGACCGAG | TCGTAGGTCTCCTTCCCTCG | Start: 256 |
| Tm | 59.968 | 60.107 | End: 454 |
| Size | 20 | 20 | 199 |
| Primer 3 details |
|---|
| Sequence | GTAAGGGCTTCGGCAGATGT | CTCGGTCTCACGGACAACAA | Start: 37 |
| Tm | 60.108 | 59.968 | End: 275 |
| Size | 20 | 20 | 239 |
| Primer 4 details |
|---|
| Sequence | TGTAAGGGCTTCGGCAGATG | CTCGGTCTCACGGACAACAA | Start: 36 |
| Tm | 60.108 | 59.968 | End: 275 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
|---|
| Sequence | TTGTTGTCCGTGAGACCGAG | GCTGAATGTACCACGCCTCT | Start: 256 |
| Tm | 59.968 | 60.108 | End: 393 |
| Size | 20 | 20 | 138 |
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