ssr_primer
Primer Details for TRINITY_DN72081_c4_g2_i2 |
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| (T)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTCTCACTCGCCGATCTG | TTCGAACACTCGTATCCGGC | Start: 878 |
| Tm | 59.829 | 60.179 | End: 1081 |
| Size | 20 | 20 | 204 |
| Primer 2 details |
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| Sequence | GGGGCTCATTGTTTGCATGG | TTTTTCCCCTGCAGCTCCTC | Start: 98 |
| Tm | 60.108 | 60.251 | End: 267 |
| Size | 20 | 20 | 170 |
| Primer 3 details |
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| Sequence | GGGGGCTCATTGTTTGCATG | TTTTTCCCCTGCAGCTCCTC | Start: 97 |
| Tm | 60.108 | 60.251 | End: 267 |
| Size | 20 | 20 | 171 |
| Primer 4 details |
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| Sequence | GAGGAGCTGCAGGGGAAAAA | GAGGACGATGGCGGAAAGAA | Start: 248 |
| Tm | 60.251 | 60.108 | End: 500 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
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| Sequence | GAGGAGCTGCAGGGGAAAAA | CGCGATCGAAAAACGGAACA | Start: 248 |
| Tm | 60.251 | 59.838 | End: 423 |
| Size | 20 | 20 | 176 |
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