ssr_primer
Primer Details for TRINITY_DN72160_c1_g1_i3 |
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| (A)10gtcaggcagatgaaggtagaatttttaaaaagagc(A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCATTCCGCCTTAAACCGCT | ACAGCGTATCACCATTCCGG | Start: 25 |
| Tm | 60.036 | 60.179 | End: 172 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
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| Sequence | CGGCCTCAAGAATTTCACGC | ACAGCGTATCACCATTCCGG | Start: 72 |
| Tm | 60.179 | 60.179 | End: 172 |
| Size | 20 | 20 | 101 |
| Primer 3 details |
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| Sequence | CCGGAATGGTGATACGCTGT | GGGAAGAAGAGTTGCGGCTA | Start: 153 |
| Tm | 60.179 | 59.751 | End: 305 |
| Size | 20 | 20 | 153 |
| Primer 4 details |
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| Sequence | CCGGAATGGTGATACGCTGT | TAGGGAAGAAGAGTTGCGGC | Start: 153 |
| Tm | 60.179 | 59.751 | End: 307 |
| Size | 20 | 20 | 155 |
| Primer 5 details |
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| Sequence | CGGCCTCAAGAATTTCACGC | GGGAAGAAGAGTTGCGGCTA | Start: 72 |
| Tm | 60.179 | 59.751 | End: 305 |
| Size | 20 | 20 | 234 |
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