ssr_primer
Primer Details for TRINITY_DN72376_c0_g1_i1 |
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| (ATTC)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTTTTGACCCACGGGCTTG | CTCCACCTGTGTATTGCGGT | Start: 263 |
| Tm | 59.968 | 60.036 | End: 519 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
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| Sequence | ACCGCAATACACAGGTGGAG | ATCTCACGTGCGTTGCAAAC | Start: 500 |
| Tm | 60.036 | 60.041 | End: 757 |
| Size | 20 | 20 | 258 |
| Primer 3 details |
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| Sequence | GTTTGCAACGCACGTGAGAT | ATTGCTCCTACGTTTCGCCA | Start: 738 |
| Tm | 60.041 | 60.037 | End: 896 |
| Size | 20 | 20 | 159 |
| Primer 4 details |
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| Sequence | GTTTGCAACGCACGTGAGAT | TCCTACGTTTCGCCAGTGAC | Start: 738 |
| Tm | 60.041 | 60.039 | End: 891 |
| Size | 20 | 20 | 154 |
| Primer 5 details |
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| Sequence | ACCGCAATACACAGGTGGAG | CCGAAACGAATCTCACGTGC | Start: 500 |
| Tm | 60.036 | 59.907 | End: 766 |
| Size | 20 | 20 | 267 |
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