Primer Details for TRINITY_DN72681_c2_g2_i1 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATCGAGGGGCTTGAAGGAC | TGTGAGTTTGAAATTTCTTACGACCA | Start: 3 |
| Tm | 59.747 | 59.683 | End: 117 |
| Size | 20 | 26 | 115 |
| Primer 2 details |
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| Sequence | AATCGAGGGGCTTGAAGGAC | GTGAGTTTGAAATTTCTTACGACCA | Start: 3 |
| Tm | 59.747 | 58.279 | End: 116 |
| Size | 20 | 25 | 114 |
| Primer 3 details |
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| Sequence | AATCGAGGGGCTTGAAGGAC | TGTGAGTTTGAAATTTCTTACGACC | Start: 3 |
| Tm | 59.747 | 58.279 | End: 117 |
| Size | 20 | 25 | 115 |
| Primer 4 details |
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| Sequence | AATCGAGGGGCTTGAAGGAC | TGAGTTTGAAATTTCTTACGACCA | Start: 3 |
| Tm | 59.747 | 57.071 | End: 115 |
| Size | 20 | 24 | 113 |
| Primer 5 details |
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| Sequence | ATCGAGGGGCTTGAAGGACA | TGTGAGTTTGAAATTTCTTACGACCA | Start: 4 |
| Tm | 60.91 | 59.683 | End: 117 |
| Size | 20 | 26 | 114 |
