ssr_primer
Primer Details for TRINITY_DN73059_c6_g2_i2 |
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| (T)25: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GATGACTTTACCGCAACCGC | GGGGGTACGTGACAATTCGT | Start: 576 |
| Tm | 59.904 | 60.037 | End: 855 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
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| Sequence | GATGTAAGGGCGCGAGTACA | GGGGGTACGTGACAATTCGT | Start: 729 |
| Tm | 59.898 | 60.037 | End: 855 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
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| Sequence | TGATGTAAGGGCGCGAGTAC | GGGGGTACGTGACAATTCGT | Start: 728 |
| Tm | 59.898 | 60.037 | End: 855 |
| Size | 20 | 20 | 128 |
| Primer 4 details |
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| Sequence | ACGGGTTCATTACGCCAAGT | CCCGGAGTGCACTAATTCGT | Start: 210 |
| Tm | 59.964 | 60.108 | End: 323 |
| Size | 20 | 20 | 114 |
| Primer 5 details |
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| Sequence | ACGAATTGTCACGTACCCCC | AGCACACAGGTTTCGGAACA | Start: 836 |
| Tm | 60.037 | 60.107 | End: 961 |
| Size | 20 | 20 | 126 |
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