ssr_primer
Primer Details for TRINITY_DN73417_c2_g1_i1 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCCCGAACCACGACTGAG | GCCGGGGTAATAGACGTGAG | Start: 146 |
| Tm | 59.967 | 59.968 | End: 378 |
| Size | 20 | 20 | 233 |
| Primer 2 details |
|---|
| Sequence | CTCACGTCTATTACCCCGGC | TAAGAGCTTGAGCCGTGTGG | Start: 359 |
| Tm | 59.968 | 60.037 | End: 567 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
|---|
| Sequence | GGCAATATAGGTCGCGACGA | AGCAACATCTTGAGGAGCCC | Start: 833 |
| Tm | 60.04 | 60.035 | End: 995 |
| Size | 20 | 20 | 163 |
| Primer 4 details |
|---|
| Sequence | GGCAATATAGGTCGCGACGA | AACATCTTGAGGAGCCCGTG | Start: 833 |
| Tm | 60.04 | 60.036 | End: 992 |
| Size | 20 | 20 | 160 |
| Primer 5 details |
|---|
| Sequence | GGCAATATAGGTCGCGACGA | CAACATCTTGAGGAGCCCGT | Start: 833 |
| Tm | 60.04 | 60.036 | End: 993 |
| Size | 20 | 20 | 161 |
(T)23cgagggagtgcgtggtttcgcgggtcgcggtgatgacgaatggggtcttggtatttag(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCCCGAACCACGACTGAG | GCCGGGGTAATAGACGTGAG | Start: 146 |
| Tm | 59.967 | 59.968 | End: 378 |
| Size | 20 | 20 | 233 |
| Primer 2 details |
|---|
| Sequence | CTCACGTCTATTACCCCGGC | TAAGAGCTTGAGCCGTGTGG | Start: 359 |
| Tm | 59.968 | 60.037 | End: 567 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
|---|
| Sequence | GGCAATATAGGTCGCGACGA | AGCAACATCTTGAGGAGCCC | Start: 833 |
| Tm | 60.04 | 60.035 | End: 995 |
| Size | 20 | 20 | 163 |
| Primer 4 details |
|---|
| Sequence | GGCAATATAGGTCGCGACGA | AACATCTTGAGGAGCCCGTG | Start: 833 |
| Tm | 60.04 | 60.036 | End: 992 |
| Size | 20 | 20 | 160 |
| Primer 5 details |
|---|
| Sequence | GGCAATATAGGTCGCGACGA | CAACATCTTGAGGAGCCCGT | Start: 833 |
| Tm | 60.04 | 60.036 | End: 993 |
| Size | 20 | 20 | 161 |
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