ssr_primer
Primer Details for TRINITY_DN73622_c0_g2_i3 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGGACTTTGGCTTCTCGG | ACGAGACAAGTTCTGGGCTG | Start: 3406 |
| Tm | 60.035 | 59.966 | End: 3557 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
|---|
| Sequence | CCGATGGGACTTTGGCTTCT | ACGAGACAAGTTCTGGGCTG | Start: 3403 |
| Tm | 60.035 | 59.966 | End: 3557 |
| Size | 20 | 20 | 155 |
| Primer 3 details |
|---|
| Sequence | GACTGGCCTTGGAAGCAGAT | CAGGAGTGAGGAAGCCCAAG | Start: 3115 |
| Tm | 60.035 | 60.036 | End: 3263 |
| Size | 20 | 20 | 149 |
| Primer 4 details |
|---|
| Sequence | CTTGGGCTTCCTCACTCCTG | AGAAGCCAAAGTCCCATCGG | Start: 3244 |
| Tm | 60.036 | 60.035 | End: 3422 |
| Size | 20 | 20 | 179 |
| Primer 5 details |
|---|
| Sequence | CTTGGGCTTCCTCACTCCTG | CCGAGAAGCCAAAGTCCCAT | Start: 3244 |
| Tm | 60.036 | 60.035 | End: 3425 |
| Size | 20 | 20 | 182 |
(A)10ttaaactgttgcaagttcatacagagagtgaaaaaaataattttcttcccaac(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGGACTTTGGCTTCTCGG | ACGAGACAAGTTCTGGGCTG | Start: 3406 |
| Tm | 60.035 | 59.966 | End: 3557 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
|---|
| Sequence | CCGATGGGACTTTGGCTTCT | ACGAGACAAGTTCTGGGCTG | Start: 3403 |
| Tm | 60.035 | 59.966 | End: 3557 |
| Size | 20 | 20 | 155 |
| Primer 3 details |
|---|
| Sequence | GACTGGCCTTGGAAGCAGAT | CAGGAGTGAGGAAGCCCAAG | Start: 3115 |
| Tm | 60.035 | 60.036 | End: 3263 |
| Size | 20 | 20 | 149 |
| Primer 4 details |
|---|
| Sequence | CTTGGGCTTCCTCACTCCTG | AGAAGCCAAAGTCCCATCGG | Start: 3244 |
| Tm | 60.036 | 60.035 | End: 3422 |
| Size | 20 | 20 | 179 |
| Primer 5 details |
|---|
| Sequence | CTTGGGCTTCCTCACTCCTG | CCGAGAAGCCAAAGTCCCAT | Start: 3244 |
| Tm | 60.036 | 60.035 | End: 3425 |
| Size | 20 | 20 | 182 |
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