ssr_primer
Primer Details for TRINITY_DN73696_c5_g2_i2 |
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| (T)29: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGTTGCCTCGTCGATACAT | ATGGATTCGCTTTCGCATGC | Start: 53 |
| Tm | 59.896 | 59.97 | End: 258 |
| Size | 20 | 20 | 206 |
| Primer 2 details |
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| Sequence | CTTCTCCCCCAAATTCGGCT | TCATGCGAGTAACGGGTAGC | Start: 1323 |
| Tm | 60.034 | 59.898 | End: 1500 |
| Size | 20 | 20 | 178 |
| Primer 3 details |
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| Sequence | TTCTCCCCCAAATTCGGCTC | TCATGCGAGTAACGGGTAGC | Start: 1324 |
| Tm | 60.034 | 59.898 | End: 1500 |
| Size | 20 | 20 | 177 |
| Primer 4 details |
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| Sequence | GTTTCCCGCAGAGACCTCAA | TCATGCGAGTAACGGGTAGC | Start: 1287 |
| Tm | 59.965 | 59.898 | End: 1500 |
| Size | 20 | 20 | 214 |
| Primer 5 details |
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| Sequence | CTTCTCCCCCAAATTCGGCT | GGATCATGCGAGTAACGGGT | Start: 1323 |
| Tm | 60.034 | 59.896 | End: 1503 |
| Size | 20 | 20 | 181 |
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