ssr_primer
Primer Details for TRINITY_DN74147_c2_g2_i4 |
|---|
| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GATGTGGCAGCAAGAGGTCT | ACTGGCTTGCAGCATACACT | Start: 345 |
| Tm | 60.036 | 59.963 | End: 590 |
| Size | 20 | 20 | 246 |
| Primer 2 details |
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| Sequence | AGCCATGGTGCATGCACTAT | ACTGGCTTGCAGCATACACT | Start: 462 |
| Tm | 60.106 | 59.963 | End: 590 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
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| Sequence | GATGTGGCAGCAAGAGGTCT | TTGACTACTGGCTTGCAGCA | Start: 345 |
| Tm | 60.036 | 59.892 | End: 596 |
| Size | 20 | 20 | 252 |
| Primer 4 details |
|---|
| Sequence | AGCCATGGTGCATGCACTAT | TTGACTACTGGCTTGCAGCA | Start: 462 |
| Tm | 60.106 | 59.892 | End: 596 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | GATGTGGCAGCAAGAGGTCT | GTGCATGCACCATGGCTTTT | Start: 345 |
| Tm | 60.036 | 60.321 | End: 478 |
| Size | 20 | 20 | 134 |
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