ssr_primer
Primer Details for TRINITY_DN74305_c1_g4_i2 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAAGCACGAACGGATCACA | CCCATTCACCGACCATCCAA | Start: 185 |
| Tm | 59.967 | 60.035 | End: 298 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
|---|
| Sequence | TCAAGCACGAACGGATCACA | CCAGTCCTCCACCATCCAAC | Start: 185 |
| Tm | 59.967 | 60.035 | End: 326 |
| Size | 20 | 20 | 142 |
| Primer 3 details |
|---|
| Sequence | TCAAGCACGAACGGATCACA | TCGTTAGCTCTCCTCCACGA | Start: 185 |
| Tm | 59.967 | 60.036 | End: 387 |
| Size | 20 | 20 | 203 |
| Primer 4 details |
|---|
| Sequence | CGTCAAACTAGGGGCTTGGT | TGTGATCCGTTCGTGCTTGA | Start: 14 |
| Tm | 59.964 | 59.967 | End: 204 |
| Size | 20 | 20 | 191 |
| Primer 5 details |
|---|
| Sequence | TGGTGAATGAGTCTGTCGCC | TGTGATCCGTTCGTGCTTGA | Start: 68 |
| Tm | 60.037 | 59.967 | End: 204 |
| Size | 20 | 20 | 137 |
(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAAGCACGAACGGATCACA | CCCATTCACCGACCATCCAA | Start: 185 |
| Tm | 59.967 | 60.035 | End: 298 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
|---|
| Sequence | TCAAGCACGAACGGATCACA | CCAGTCCTCCACCATCCAAC | Start: 185 |
| Tm | 59.967 | 60.035 | End: 326 |
| Size | 20 | 20 | 142 |
| Primer 3 details |
|---|
| Sequence | TCAAGCACGAACGGATCACA | TCGTTAGCTCTCCTCCACGA | Start: 185 |
| Tm | 59.967 | 60.036 | End: 387 |
| Size | 20 | 20 | 203 |
| Primer 4 details |
|---|
| Sequence | CGTCAAACTAGGGGCTTGGT | TGTGATCCGTTCGTGCTTGA | Start: 14 |
| Tm | 59.964 | 59.967 | End: 204 |
| Size | 20 | 20 | 191 |
| Primer 5 details |
|---|
| Sequence | TGGTGAATGAGTCTGTCGCC | TGTGATCCGTTCGTGCTTGA | Start: 68 |
| Tm | 60.037 | 59.967 | End: 204 |
| Size | 20 | 20 | 137 |
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