ssr_primer
Primer Details for TRINITY_DN74421_c5_g5_i1 |
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| (T)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGGGATAATTGCACTCGC | AGGAACCAGCGCGATTACAA | Start: 3 |
| Tm | 60.465 | 60.037 | End: 211 |
| Size | 20 | 20 | 209 |
| Primer 2 details |
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| Sequence | TGGGGGATAATTGCACTCGC | AAGGAACCAGCGCGATTACA | Start: 3 |
| Tm | 60.465 | 60.037 | End: 212 |
| Size | 20 | 20 | 210 |
| Primer 3 details |
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| Sequence | TGGGGGATAATTGCACTCGC | CAAAAAGGAACCAGCGCGAT | Start: 3 |
| Tm | 60.465 | 59.759 | End: 216 |
| Size | 20 | 20 | 214 |
| Primer 4 details |
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| Sequence | CGCACAAAGTAATAGCGCCT | AGGAACCAGCGCGATTACAA | Start: 20 |
| Tm | 58.993 | 60.037 | End: 211 |
| Size | 20 | 20 | 192 |
| Primer 5 details |
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| Sequence | CGCACAAAGTAATAGCGCCT | AAGGAACCAGCGCGATTACA | Start: 20 |
| Tm | 58.993 | 60.037 | End: 212 |
| Size | 20 | 20 | 193 |
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