ssr_primer
Primer Details for TRINITY_DN74466_c0_g5_i1 |
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| (T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGTGCGTATACTTTGCCGC | TTGAGAGGAGAAAGCTGCGG | Start: 702 |
| Tm | 59.974 | 60.037 | End: 830 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
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| Sequence | CCGCAGCTTTCTCCTCTCAA | GGATCACGTAGAGAAGGGCG | Start: 811 |
| Tm | 60.037 | 59.969 | End: 992 |
| Size | 20 | 20 | 182 |
| Primer 3 details |
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| Sequence | ATTTTTACACGCAACCCGCC | GGATCACGTAGAGAAGGGCG | Start: 854 |
| Tm | 60.039 | 59.969 | End: 992 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
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| Sequence | CCGCAGCTTTCTCCTCTCAA | GGGCCTTCATCGTCTGGAAA | Start: 811 |
| Tm | 60.037 | 60.035 | End: 1031 |
| Size | 20 | 20 | 221 |
| Primer 5 details |
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| Sequence | ATTTTTACACGCAACCCGCC | GGGCCTTCATCGTCTGGAAA | Start: 854 |
| Tm | 60.039 | 60.035 | End: 1031 |
| Size | 20 | 20 | 178 |
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