ssr_primer
Primer Details for TRINITY_DN74642_c3_g2_i2 |
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| (T)23gaaaaaccagtgtaagttttttgttgaattatgaaaatcggattgattgacccaaatggataacagtgaatttttttt(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCGCTCTTCCGTTTCTTTG | TGCGTCCATCCCCTTGTAAC | Start: 139 |
| Tm | 60.11 | 60.036 | End: 272 |
| Size | 20 | 20 | 134 |
| Primer 2 details |
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| Sequence | TCTTTGCTTCCCACCTTCCC | TGCGTCCATCCCCTTGTAAC | Start: 153 |
| Tm | 59.888 | 60.036 | End: 272 |
| Size | 20 | 20 | 120 |
| Primer 3 details |
|---|
| Sequence | GGCGCTCTTCCGTTTCTTTG | AAATGCGTCCATCCCCTTGT | Start: 139 |
| Tm | 60.11 | 59.96 | End: 275 |
| Size | 20 | 20 | 137 |
| Primer 4 details |
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| Sequence | TCTTTGCTTCCCACCTTCCC | AAATGCGTCCATCCCCTTGT | Start: 153 |
| Tm | 59.888 | 59.96 | End: 275 |
| Size | 20 | 20 | 123 |
| Primer 5 details |
|---|
| Sequence | ACGTAACGCTTGGAGGGAAG | TTCGAAAAATCTGCGCGACG | Start: 218 |
| Tm | 60.038 | 60.179 | End: 328 |
| Size | 20 | 20 | 111 |
(T)23: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCGCTCTTCCGTTTCTTTG | TGCGTCCATCCCCTTGTAAC | Start: 139 |
| Tm | 60.11 | 60.036 | End: 272 |
| Size | 20 | 20 | 134 |
| Primer 2 details |
|---|
| Sequence | TCTTTGCTTCCCACCTTCCC | TGCGTCCATCCCCTTGTAAC | Start: 153 |
| Tm | 59.888 | 60.036 | End: 272 |
| Size | 20 | 20 | 120 |
| Primer 3 details |
|---|
| Sequence | GGCGCTCTTCCGTTTCTTTG | AAATGCGTCCATCCCCTTGT | Start: 139 |
| Tm | 60.11 | 59.96 | End: 275 |
| Size | 20 | 20 | 137 |
| Primer 4 details |
|---|
| Sequence | TCTTTGCTTCCCACCTTCCC | AAATGCGTCCATCCCCTTGT | Start: 153 |
| Tm | 59.888 | 59.96 | End: 275 |
| Size | 20 | 20 | 123 |
| Primer 5 details |
|---|
| Sequence | ACGTAACGCTTGGAGGGAAG | TTCGAAAAATCTGCGCGACG | Start: 218 |
| Tm | 60.038 | 60.179 | End: 328 |
| Size | 20 | 20 | 111 |
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