ssr_primer
Primer Details for TRINITY_DN74668_c3_g2_i6 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCTACGTACAAAATGCGGG | CCCAGATTTCGCGGGTAGTT | Start: 252 |
| Tm | 59.974 | 60.108 | End: 491 |
| Size | 20 | 20 | 240 |
| Primer 2 details |
|---|
| Sequence | CGCTACGTACAAAATGCGGG | TCGCGGGTAGTTGTTACGTC | Start: 252 |
| Tm | 59.974 | 60.11 | End: 483 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
|---|
| Sequence | TCGTGCTTTATGGGTGTGCT | TGAGTTGCGTGGTTTTGCAG | Start: 627 |
| Tm | 59.964 | 59.9 | End: 802 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
|---|
| Sequence | TCGTGCTTTATGGGTGTGCT | GAGTTGCGTGGTTTTGCAGT | Start: 627 |
| Tm | 59.964 | 59.899 | End: 801 |
| Size | 20 | 20 | 175 |
| Primer 5 details |
|---|
| Sequence | TCGTGCTTTATGGGTGTGCT | AGTGAGTTGCGTGGTTTTGC | Start: 627 |
| Tm | 59.964 | 59.899 | End: 804 |
| Size | 20 | 20 | 178 |
(A)17cctggaaaagtaccctgatatatacgcacctaatatacggttac(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCTACGTACAAAATGCGGG | CCCAGATTTCGCGGGTAGTT | Start: 252 |
| Tm | 59.974 | 60.108 | End: 491 |
| Size | 20 | 20 | 240 |
| Primer 2 details |
|---|
| Sequence | CGCTACGTACAAAATGCGGG | TCGCGGGTAGTTGTTACGTC | Start: 252 |
| Tm | 59.974 | 60.11 | End: 483 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
|---|
| Sequence | TCGTGCTTTATGGGTGTGCT | TGAGTTGCGTGGTTTTGCAG | Start: 627 |
| Tm | 59.964 | 59.9 | End: 802 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
|---|
| Sequence | TCGTGCTTTATGGGTGTGCT | GAGTTGCGTGGTTTTGCAGT | Start: 627 |
| Tm | 59.964 | 59.899 | End: 801 |
| Size | 20 | 20 | 175 |
| Primer 5 details |
|---|
| Sequence | TCGTGCTTTATGGGTGTGCT | AGTGAGTTGCGTGGTTTTGC | Start: 627 |
| Tm | 59.964 | 59.899 | End: 804 |
| Size | 20 | 20 | 178 |
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