ssr_primer
Primer Details for TRINITY_DN74989_c2_g1_i4 |
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| (T)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATTGCTCATCAGCCCAGCT | GTACGGTTGAGCATTGGGGA | Start: 111 |
| Tm | 60.033 | 60.036 | End: 253 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
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| Sequence | GCAGGAAGGCAAACCAACAG | GAGTGAACAGAAGGGTCCCG | Start: 523 |
| Tm | 59.967 | 60.037 | End: 786 |
| Size | 20 | 20 | 264 |
| Primer 3 details |
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| Sequence | TCATCAGCCCAGCTCAAAGG | GTACGGTTGAGCATTGGGGA | Start: 117 |
| Tm | 60.035 | 60.036 | End: 253 |
| Size | 20 | 20 | 137 |
| Primer 4 details |
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| Sequence | GAATGGCAGTGGGACAGACA | GAGTGAACAGAAGGGTCCCG | Start: 544 |
| Tm | 59.963 | 60.037 | End: 786 |
| Size | 20 | 20 | 243 |
| Primer 5 details |
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| Sequence | TGAATGGCAGTGGGACAGAC | GAGTGAACAGAAGGGTCCCG | Start: 543 |
| Tm | 59.963 | 60.037 | End: 786 |
| Size | 20 | 20 | 244 |
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