ssr_primer
Primer Details for TRINITY_DN75434_c4_g2_i6 |
|---|
| (AAAG)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGATACGCACGCTTGAACTG | GCATCGTTGGAGGAAGGTCA | Start: 71 |
| Tm | 59.976 | 60.036 | End: 346 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | TAACGCGCGCCAAAATATCG | GTTTTCTCCCCCTTCCCCAG | Start: 813 |
| Tm | 60.043 | 59.96 | End: 938 |
| Size | 20 | 20 | 126 |
| Primer 3 details |
|---|
| Sequence | TAACGCGCGCCAAAATATCG | CTGGGTTTTCTCCCCCTTCC | Start: 813 |
| Tm | 60.043 | 59.96 | End: 942 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
|---|
| Sequence | CGATACGCACGCTTGAACTG | CGTTGGAGGAAGGTCAACCA | Start: 71 |
| Tm | 59.976 | 59.892 | End: 342 |
| Size | 20 | 20 | 272 |
| Primer 5 details |
|---|
| Sequence | AATGTAACGCGCGCCAAAAT | GTTTTCTCCCCCTTCCCCAG | Start: 809 |
| Tm | 60.11 | 59.96 | End: 938 |
| Size | 20 | 20 | 130 |
(A)22: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGATACGCACGCTTGAACTG | GCATCGTTGGAGGAAGGTCA | Start: 71 |
| Tm | 59.976 | 60.036 | End: 346 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | TAACGCGCGCCAAAATATCG | GTTTTCTCCCCCTTCCCCAG | Start: 813 |
| Tm | 60.043 | 59.96 | End: 938 |
| Size | 20 | 20 | 126 |
| Primer 3 details |
|---|
| Sequence | TAACGCGCGCCAAAATATCG | CTGGGTTTTCTCCCCCTTCC | Start: 813 |
| Tm | 60.043 | 59.96 | End: 942 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
|---|
| Sequence | CGATACGCACGCTTGAACTG | CGTTGGAGGAAGGTCAACCA | Start: 71 |
| Tm | 59.976 | 59.892 | End: 342 |
| Size | 20 | 20 | 272 |
| Primer 5 details |
|---|
| Sequence | AATGTAACGCGCGCCAAAAT | GTTTTCTCCCCCTTCCCCAG | Start: 809 |
| Tm | 60.11 | 59.96 | End: 938 |
| Size | 20 | 20 | 130 |
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