Primer Details for TRINITY_DN75719_c7_g5_i1 |
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| (TTC)6cttttcttctttttctacttcttccttttctta(T)20c(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAAACTGTTCTTCCTTCTCCTCCT | AAGGAAAAGGAGAGAGCAAGGG | Start: 30 |
| Tm | 60.104 | 59.959 | End: 129 |
| Size | 25 | 22 | 100 |
| Primer 2 details |
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| Sequence | AAAAACTGTTCTTCCTTCTCCTCC | AAGGAAAAGGAGAGAGCAAGGG | Start: 30 |
| Tm | 58.866 | 59.959 | End: 129 |
| Size | 24 | 22 | 100 |
| Primer 3 details |
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| Sequence | TAAAAACTGTTCTTCCTTCTCCTCCT | AGGAAAAGGAGAGAGCAAGGG | Start: 29 |
| Tm | 59.899 | 59.367 | End: 128 |
| Size | 26 | 21 | 100 |
| Primer 4 details |
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| Sequence | AAAACTGTTCTTCCTTCTCCTCCT | AAAGGAAAAGGAGAGAGCAAGGG | Start: 31 |
| Tm | 59.586 | 60.499 | End: 130 |
| Size | 24 | 23 | 100 |
| Primer 5 details |
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| Sequence | TAAAAACTGTTCTTCCTTCTCCTCC | AGGAAAAGGAGAGAGCAAGGG | Start: 29 |
| Tm | 58.703 | 59.367 | End: 128 |
| Size | 25 | 21 | 100 |
