ssr_primer
Primer Details for TRINITY_DN76120_c1_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGACTTACGAGCGAACGTG | ATCACCGCTAAGCAGCTGTT | Start: 369 |
| Tm | 60.11 | 60.036 | End: 647 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
|---|
| Sequence | GCAATCACGTGCGTGCTTAA | CTTGCGACAGAGAAGCAACG | Start: 660 |
| Tm | 60.11 | 59.837 | End: 922 |
| Size | 20 | 20 | 263 |
| Primer 3 details |
|---|
| Sequence | AGGACTTACGAGCGAACGTG | ACCGCTAAGCAGCTGTTGAA | Start: 369 |
| Tm | 60.11 | 60.25 | End: 644 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
|---|
| Sequence | TGAGGACTTACGAGCGAACG | ACCGCTAAGCAGCTGTTGAA | Start: 367 |
| Tm | 59.833 | 60.25 | End: 644 |
| Size | 20 | 20 | 278 |
| Primer 5 details |
|---|
| Sequence | GGACTTACGAGCGAACGTGT | ATCACCGCTAAGCAGCTGTT | Start: 370 |
| Tm | 60.386 | 60.036 | End: 647 |
| Size | 20 | 20 | 278 |
(T)19: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGACTTACGAGCGAACGTG | ATCACCGCTAAGCAGCTGTT | Start: 369 |
| Tm | 60.11 | 60.036 | End: 647 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
|---|
| Sequence | GCAATCACGTGCGTGCTTAA | CTTGCGACAGAGAAGCAACG | Start: 660 |
| Tm | 60.11 | 59.837 | End: 922 |
| Size | 20 | 20 | 263 |
| Primer 3 details |
|---|
| Sequence | AGGACTTACGAGCGAACGTG | ACCGCTAAGCAGCTGTTGAA | Start: 369 |
| Tm | 60.11 | 60.25 | End: 644 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
|---|
| Sequence | TGAGGACTTACGAGCGAACG | ACCGCTAAGCAGCTGTTGAA | Start: 367 |
| Tm | 59.833 | 60.25 | End: 644 |
| Size | 20 | 20 | 278 |
| Primer 5 details |
|---|
| Sequence | GGACTTACGAGCGAACGTGT | ATCACCGCTAAGCAGCTGTT | Start: 370 |
| Tm | 60.386 | 60.036 | End: 647 |
| Size | 20 | 20 | 278 |
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