ssr_primer
Primer Details for TRINITY_DN76336_c2_g4_i4 |
|---|
| (G)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGTTACACCGGTTTTGGGG | TATGCCGACTCCCAATGAGC | Start: 841 |
| Tm | 60.179 | 59.893 | End: 1110 |
| Size | 20 | 20 | 270 |
| Primer 2 details |
|---|
| Sequence | GCTCATTGGGAGTCGGCATA | GCACCTCAACTGCACTGTTC | Start: 1091 |
| Tm | 59.893 | 59.691 | End: 1222 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | GCTCATTGGGAGTCGGCATA | TGCACCTCAACTGCACTGTT | Start: 1091 |
| Tm | 59.893 | 60.394 | End: 1223 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
|---|
| Sequence | GCTCATTGGGAGTCGGCATA | TTGCACCTCAACTGCACTGT | Start: 1091 |
| Tm | 59.893 | 60.394 | End: 1224 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
|---|
| Sequence | GCTCATTGGGAGTCGGCATA | ACCTCAACTGCACTGTTCCA | Start: 1091 |
| Tm | 59.893 | 59.454 | End: 1220 |
| Size | 20 | 20 | 130 |
(AT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGTTACACCGGTTTTGGGG | TATGCCGACTCCCAATGAGC | Start: 841 |
| Tm | 60.179 | 59.893 | End: 1110 |
| Size | 20 | 20 | 270 |
| Primer 2 details |
|---|
| Sequence | GCTCATTGGGAGTCGGCATA | GCACCTCAACTGCACTGTTC | Start: 1091 |
| Tm | 59.893 | 59.691 | End: 1222 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | GCTCATTGGGAGTCGGCATA | TGCACCTCAACTGCACTGTT | Start: 1091 |
| Tm | 59.893 | 60.394 | End: 1223 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
|---|
| Sequence | GCTCATTGGGAGTCGGCATA | TTGCACCTCAACTGCACTGT | Start: 1091 |
| Tm | 59.893 | 60.394 | End: 1224 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
|---|
| Sequence | GCTCATTGGGAGTCGGCATA | ACCTCAACTGCACTGTTCCA | Start: 1091 |
| Tm | 59.893 | 59.454 | End: 1220 |
| Size | 20 | 20 | 130 |
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