ssr_primer
Primer Details for TRINITY_DN76367_c5_g3_i2 |
|---|
| (T)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTACATTCCTGGTGGCCCC | CGACGACTCCCGAAAAGACA | Start: 749 |
| Tm | 59.96 | 60.04 | End: 971 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
|---|
| Sequence | AAAAGCTGAGAGTTGGCCGA | CGACGACTCCCGAAAAGACA | Start: 798 |
| Tm | 59.891 | 60.04 | End: 971 |
| Size | 20 | 20 | 174 |
| Primer 3 details |
|---|
| Sequence | CGAGGCGAGACACCTGAAAT | GGGGCCACCAGGAATGTAAA | Start: 576 |
| Tm | 60.109 | 59.96 | End: 768 |
| Size | 20 | 20 | 193 |
| Primer 4 details |
|---|
| Sequence | TTTACATTCCTGGTGGCCCC | GTTAGCGACGACTCCCGAAA | Start: 749 |
| Tm | 59.96 | 60.11 | End: 976 |
| Size | 20 | 20 | 228 |
| Primer 5 details |
|---|
| Sequence | TTTACATTCCTGGTGGCCCC | TAGCGACGACTCCCGAAAAG | Start: 749 |
| Tm | 59.96 | 59.83 | End: 974 |
| Size | 20 | 20 | 226 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTACATTCCTGGTGGCCCC | CGACGACTCCCGAAAAGACA | Start: 749 |
| Tm | 59.96 | 60.04 | End: 971 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
|---|
| Sequence | AAAAGCTGAGAGTTGGCCGA | CGACGACTCCCGAAAAGACA | Start: 798 |
| Tm | 59.891 | 60.04 | End: 971 |
| Size | 20 | 20 | 174 |
| Primer 3 details |
|---|
| Sequence | CGAGGCGAGACACCTGAAAT | GGGGCCACCAGGAATGTAAA | Start: 576 |
| Tm | 60.109 | 59.96 | End: 768 |
| Size | 20 | 20 | 193 |
| Primer 4 details |
|---|
| Sequence | TTTACATTCCTGGTGGCCCC | GTTAGCGACGACTCCCGAAA | Start: 749 |
| Tm | 59.96 | 60.11 | End: 976 |
| Size | 20 | 20 | 228 |
| Primer 5 details |
|---|
| Sequence | TTTACATTCCTGGTGGCCCC | TAGCGACGACTCCCGAAAAG | Start: 749 |
| Tm | 59.96 | 59.83 | End: 974 |
| Size | 20 | 20 | 226 |
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