ssr_primer
Primer Details for TRINITY_DN76477_c3_g1_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGCTTTTACGCCATGAGTTG | ACAGTCAAACGGGTGGGTTT | Start: 421 |
| Tm | 59.803 | 60.034 | End: 560 |
| Size | 21 | 20 | 140 |
| Primer 2 details |
|---|
| Sequence | ACGCTTTTACGCCATGAGTTG | AGCTACAGTCAAACGGGTGG | Start: 421 |
| Tm | 59.803 | 59.965 | End: 564 |
| Size | 21 | 20 | 144 |
| Primer 3 details |
|---|
| Sequence | AATAAGTGCCACCCAGCGAA | CAACTCATGGCGTAAAAGCGT | Start: 244 |
| Tm | 59.962 | 59.803 | End: 441 |
| Size | 20 | 21 | 198 |
| Primer 4 details |
|---|
| Sequence | CCCCCTCCCTTTTATCACCA | ACAGTCAAACGGGTGGGTTT | Start: 333 |
| Tm | 58.695 | 60.034 | End: 560 |
| Size | 20 | 20 | 228 |
| Primer 5 details |
|---|
| Sequence | CCCCCTCCCTTTTATCACCA | AGCTACAGTCAAACGGGTGG | Start: 333 |
| Tm | 58.695 | 59.965 | End: 564 |
| Size | 20 | 20 | 232 |
(C)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGCTTTTACGCCATGAGTTG | ACAGTCAAACGGGTGGGTTT | Start: 421 |
| Tm | 59.803 | 60.034 | End: 560 |
| Size | 21 | 20 | 140 |
| Primer 2 details |
|---|
| Sequence | ACGCTTTTACGCCATGAGTTG | AGCTACAGTCAAACGGGTGG | Start: 421 |
| Tm | 59.803 | 59.965 | End: 564 |
| Size | 21 | 20 | 144 |
| Primer 3 details |
|---|
| Sequence | AATAAGTGCCACCCAGCGAA | CAACTCATGGCGTAAAAGCGT | Start: 244 |
| Tm | 59.962 | 59.803 | End: 441 |
| Size | 20 | 21 | 198 |
| Primer 4 details |
|---|
| Sequence | CCCCCTCCCTTTTATCACCA | ACAGTCAAACGGGTGGGTTT | Start: 333 |
| Tm | 58.695 | 60.034 | End: 560 |
| Size | 20 | 20 | 228 |
| Primer 5 details |
|---|
| Sequence | CCCCCTCCCTTTTATCACCA | AGCTACAGTCAAACGGGTGG | Start: 333 |
| Tm | 58.695 | 59.965 | End: 564 |
| Size | 20 | 20 | 232 |
(A)11tttaatgtgaggtttttaaaaaataaaaaattatttttttcatttgtgtaaaatttatttttc(A)29: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGCTTTTACGCCATGAGTTG | ACAGTCAAACGGGTGGGTTT | Start: 421 |
| Tm | 59.803 | 60.034 | End: 560 |
| Size | 21 | 20 | 140 |
| Primer 2 details |
|---|
| Sequence | ACGCTTTTACGCCATGAGTTG | AGCTACAGTCAAACGGGTGG | Start: 421 |
| Tm | 59.803 | 59.965 | End: 564 |
| Size | 21 | 20 | 144 |
| Primer 3 details |
|---|
| Sequence | AATAAGTGCCACCCAGCGAA | CAACTCATGGCGTAAAAGCGT | Start: 244 |
| Tm | 59.962 | 59.803 | End: 441 |
| Size | 20 | 21 | 198 |
| Primer 4 details |
|---|
| Sequence | CCCCCTCCCTTTTATCACCA | ACAGTCAAACGGGTGGGTTT | Start: 333 |
| Tm | 58.695 | 60.034 | End: 560 |
| Size | 20 | 20 | 228 |
| Primer 5 details |
|---|
| Sequence | CCCCCTCCCTTTTATCACCA | AGCTACAGTCAAACGGGTGG | Start: 333 |
| Tm | 58.695 | 59.965 | End: 564 |
| Size | 20 | 20 | 232 |
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