ssr_primer
Primer Details for TRINITY_DN76498_c3_g1_i2 |
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| (T)25: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCGCATTTCGAGACTTTT | GTTTGGCTGCCCTTTCATGG | Start: 278 |
| Tm | 59.834 | 60.037 | End: 417 |
| Size | 20 | 20 | 140 |
| Primer 2 details |
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| Sequence | CAATTAGCGGCCAACAGACG | AAAAGTCTCGAAATGCGCCG | Start: 98 |
| Tm | 59.903 | 59.834 | End: 297 |
| Size | 20 | 20 | 200 |
| Primer 3 details |
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| Sequence | GGGCGTCTCGGAAAGAATCA | AAAAGTCTCGAAATGCGCCG | Start: 44 |
| Tm | 60.108 | 59.834 | End: 297 |
| Size | 20 | 20 | 254 |
| Primer 4 details |
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| Sequence | ACAAAATCCAATTCGCGGCG | GTTTGGCTGCCCTTTCATGG | Start: 263 |
| Tm | 60.456 | 60.037 | End: 417 |
| Size | 20 | 20 | 155 |
| Primer 5 details |
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| Sequence | CAATTAGCGGCCAACAGACG | CGCCGCGAATTGGATTTTGT | Start: 98 |
| Tm | 59.903 | 60.456 | End: 282 |
| Size | 20 | 20 | 185 |
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