ssr_primer
Primer Details for TRINITY_DN76985_c0_g2_i7 |
|---|
| (T)17: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCTGCCACTCAATGCTGG | GTAACCGGGATGGAGCGAAT | Start: 627 |
| Tm | 60.036 | 59.894 | End: 749 |
| Size | 20 | 20 | 123 |
| Primer 2 details |
|---|
| Sequence | TGCCACTCAATGCTGGGATT | GTAACCGGGATGGAGCGAAT | Start: 631 |
| Tm | 59.96 | 59.894 | End: 749 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
|---|
| Sequence | ACGTTATTATTCCCCGCGCA | CTGAGGGCAAATGCTACCGA | Start: 2110 |
| Tm | 60.179 | 60.108 | End: 2382 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
|---|
| Sequence | TCTCTGCCACTCAATGCTGG | ACAAGTAACCGGGATGGAGC | Start: 627 |
| Tm | 60.036 | 59.748 | End: 753 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | TGCCACTCAATGCTGGGATT | ACAAGTAACCGGGATGGAGC | Start: 631 |
| Tm | 59.96 | 59.748 | End: 753 |
| Size | 20 | 20 | 123 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCTGCCACTCAATGCTGG | GTAACCGGGATGGAGCGAAT | Start: 627 |
| Tm | 60.036 | 59.894 | End: 749 |
| Size | 20 | 20 | 123 |
| Primer 2 details |
|---|
| Sequence | TGCCACTCAATGCTGGGATT | GTAACCGGGATGGAGCGAAT | Start: 631 |
| Tm | 59.96 | 59.894 | End: 749 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
|---|
| Sequence | ACGTTATTATTCCCCGCGCA | CTGAGGGCAAATGCTACCGA | Start: 2110 |
| Tm | 60.179 | 60.108 | End: 2382 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
|---|
| Sequence | TCTCTGCCACTCAATGCTGG | ACAAGTAACCGGGATGGAGC | Start: 627 |
| Tm | 60.036 | 59.748 | End: 753 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | TGCCACTCAATGCTGGGATT | ACAAGTAACCGGGATGGAGC | Start: 631 |
| Tm | 59.96 | 59.748 | End: 753 |
| Size | 20 | 20 | 123 |
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