ssr_primer
Primer Details for TRINITY_DN76993_c0_g2_i2 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAGAAGGCTGGACAGGACC | AGATTGCAGGCATTCGGTGA | Start: 140 |
| Tm | 60.036 | 60.035 | End: 293 |
| Size | 20 | 20 | 154 |
| Primer 2 details |
|---|
| Sequence | TCCCTGCAAGCATGTGTGAT | GCAGCACTGAGAGCCTAGAG | Start: 1436 |
| Tm | 59.961 | 59.897 | End: 1559 |
| Size | 20 | 20 | 124 |
| Primer 3 details |
|---|
| Sequence | ATGGACAGCAGAATCGGGTG | AGATTGCAGGCATTCGGTGA | Start: 193 |
| Tm | 60.108 | 60.035 | End: 293 |
| Size | 20 | 20 | 101 |
| Primer 4 details |
|---|
| Sequence | TCACCGAATGCCTGCAATCT | TGCCCTCTTTTCAGCCAGTT | Start: 274 |
| Tm | 60.035 | 59.814 | End: 522 |
| Size | 20 | 20 | 249 |
| Primer 5 details |
|---|
| Sequence | TCACCGAATGCCTGCAATCT | TTGCCCTCTTTTCAGCCAGT | Start: 274 |
| Tm | 60.035 | 59.814 | End: 523 |
| Size | 20 | 20 | 250 |
(T)23: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAGAAGGCTGGACAGGACC | AGATTGCAGGCATTCGGTGA | Start: 140 |
| Tm | 60.036 | 60.035 | End: 293 |
| Size | 20 | 20 | 154 |
| Primer 2 details |
|---|
| Sequence | TCCCTGCAAGCATGTGTGAT | GCAGCACTGAGAGCCTAGAG | Start: 1436 |
| Tm | 59.961 | 59.897 | End: 1559 |
| Size | 20 | 20 | 124 |
| Primer 3 details |
|---|
| Sequence | ATGGACAGCAGAATCGGGTG | AGATTGCAGGCATTCGGTGA | Start: 193 |
| Tm | 60.108 | 60.035 | End: 293 |
| Size | 20 | 20 | 101 |
| Primer 4 details |
|---|
| Sequence | TCACCGAATGCCTGCAATCT | TGCCCTCTTTTCAGCCAGTT | Start: 274 |
| Tm | 60.035 | 59.814 | End: 522 |
| Size | 20 | 20 | 249 |
| Primer 5 details |
|---|
| Sequence | TCACCGAATGCCTGCAATCT | TTGCCCTCTTTTCAGCCAGT | Start: 274 |
| Tm | 60.035 | 59.814 | End: 523 |
| Size | 20 | 20 | 250 |
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