ssr_primer
Primer Details for TRINITY_DN77237_c3_g1_i1 |
|---|
| (CAG)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGCAGAGACCTGAAACAG | CAGGTCTCTGCATGGACACC | Start: 127 |
| Tm | 59.966 | 60.392 | End: 227 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | ACCTTAGGTGCGGGAACAAA | CGTACCGGGATCGTCGATTT | Start: 7 |
| Tm | 59.525 | 59.97 | End: 205 |
| Size | 20 | 20 | 199 |
| Primer 3 details |
|---|
| Sequence | ACCTTAGGTGCGGGAACAAA | CTGTTTCAGGTCTCTGCCGT | Start: 7 |
| Tm | 59.525 | 59.966 | End: 146 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | GAACAAATCCAGCAGCAGCA | CGTACCGGGATCGTCGATTT | Start: 20 |
| Tm | 59.4 | 59.97 | End: 205 |
| Size | 20 | 20 | 186 |
| Primer 5 details |
|---|
| Sequence | AACAAATCCAGCAGCAGCAG | CGTACCGGGATCGTCGATTT | Start: 21 |
| Tm | 59.398 | 59.97 | End: 205 |
| Size | 20 | 20 | 185 |
(A)12tcgacgatcccggtacgacggtgtccatgcagagacctgaaacagagaataaaaaaagaaaaaaaaagaatag(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGCAGAGACCTGAAACAG | CAGGTCTCTGCATGGACACC | Start: 127 |
| Tm | 59.966 | 60.392 | End: 227 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | ACCTTAGGTGCGGGAACAAA | CGTACCGGGATCGTCGATTT | Start: 7 |
| Tm | 59.525 | 59.97 | End: 205 |
| Size | 20 | 20 | 199 |
| Primer 3 details |
|---|
| Sequence | ACCTTAGGTGCGGGAACAAA | CTGTTTCAGGTCTCTGCCGT | Start: 7 |
| Tm | 59.525 | 59.966 | End: 146 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | GAACAAATCCAGCAGCAGCA | CGTACCGGGATCGTCGATTT | Start: 20 |
| Tm | 59.4 | 59.97 | End: 205 |
| Size | 20 | 20 | 186 |
| Primer 5 details |
|---|
| Sequence | AACAAATCCAGCAGCAGCAG | CGTACCGGGATCGTCGATTT | Start: 21 |
| Tm | 59.398 | 59.97 | End: 205 |
| Size | 20 | 20 | 185 |
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