ssr_primer
Primer Details for TRINITY_DN77271_c1_g1_i17 |
|---|
| (A)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCAGCTTCCTGTGCAATT | GGTGCAAGACGTCTCTGTGA | Start: 1799 |
| Tm | 60.179 | 59.968 | End: 2054 |
| Size | 20 | 20 | 256 |
| Primer 2 details |
|---|
| Sequence | TTGGTCTGTGAGCGAACTCC | AATTGCACAGGAAGCTGGGT | Start: 1570 |
| Tm | 59.966 | 60.179 | End: 1818 |
| Size | 20 | 20 | 249 |
| Primer 3 details |
|---|
| Sequence | TTTAGCTGTGCCGGGAGATG | ACTGTGGAATGGCATTGGGT | Start: 534 |
| Tm | 60.108 | 59.885 | End: 674 |
| Size | 20 | 20 | 141 |
| Primer 4 details |
|---|
| Sequence | ACCCAATGCCATTCCACAGT | TAGGTGCGTGCTCCTTTTGT | Start: 655 |
| Tm | 59.885 | 59.892 | End: 872 |
| Size | 20 | 20 | 218 |
| Primer 5 details |
|---|
| Sequence | TTTAGCTGTGCCGGGAGATG | TCAGCAACTGTGGAATGGCA | Start: 534 |
| Tm | 60.108 | 60.179 | End: 680 |
| Size | 20 | 20 | 147 |
(A)23: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCAGCTTCCTGTGCAATT | GGTGCAAGACGTCTCTGTGA | Start: 1799 |
| Tm | 60.179 | 59.968 | End: 2054 |
| Size | 20 | 20 | 256 |
| Primer 2 details |
|---|
| Sequence | TTGGTCTGTGAGCGAACTCC | AATTGCACAGGAAGCTGGGT | Start: 1570 |
| Tm | 59.966 | 60.179 | End: 1818 |
| Size | 20 | 20 | 249 |
| Primer 3 details |
|---|
| Sequence | TTTAGCTGTGCCGGGAGATG | ACTGTGGAATGGCATTGGGT | Start: 534 |
| Tm | 60.108 | 59.885 | End: 674 |
| Size | 20 | 20 | 141 |
| Primer 4 details |
|---|
| Sequence | ACCCAATGCCATTCCACAGT | TAGGTGCGTGCTCCTTTTGT | Start: 655 |
| Tm | 59.885 | 59.892 | End: 872 |
| Size | 20 | 20 | 218 |
| Primer 5 details |
|---|
| Sequence | TTTAGCTGTGCCGGGAGATG | TCAGCAACTGTGGAATGGCA | Start: 534 |
| Tm | 60.108 | 60.179 | End: 680 |
| Size | 20 | 20 | 147 |
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