ssr_primer
Primer Details for TRINITY_DN77366_c3_g1_i2 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCCCTCCCCCTTTTCCTA | TACATACGTTGGTGCTGGCA | Start: 727 |
| Tm | 59.954 | 59.678 | End: 877 |
| Size | 20 | 20 | 151 |
| Primer 2 details |
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| Sequence | GCAGCGTTGCTCATTGATGA | TAGGAAAAGGGGGAGGGAGG | Start: 539 |
| Tm | 59.551 | 59.954 | End: 746 |
| Size | 20 | 20 | 208 |
| Primer 3 details |
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| Sequence | TCTCTGTGGAGTCGATTCGC | TAGGAAAAGGGGGAGGGAGG | Start: 626 |
| Tm | 59.548 | 59.954 | End: 746 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
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| Sequence | CTCTGTGGAGTCGATTCGCT | TAGGAAAAGGGGGAGGGAGG | Start: 627 |
| Tm | 59.546 | 59.954 | End: 746 |
| Size | 20 | 20 | 120 |
| Primer 5 details |
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| Sequence | CCTCCCTCCCCCTTTTCCTA | TACGTTGGTGCTGGCAAAGT | Start: 727 |
| Tm | 59.954 | 60.465 | End: 873 |
| Size | 20 | 20 | 147 |
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